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首頁> 外文期刊>Biochimica et Biophysica Acta. Molecular and cell biology of Lipids >The activities of monocyte lysophosphatidylcholine acyltransferase and coenzyme A-independent transacylase are changed by the inflammatory cytokines tumor necrosis factor alpha and interferon gamma
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The activities of monocyte lysophosphatidylcholine acyltransferase and coenzyme A-independent transacylase are changed by the inflammatory cytokines tumor necrosis factor alpha and interferon gamma

機譯：炎性細胞因子腫瘤壞死因子α和干擾素γ改變了單核細胞溶血磷脂酰膽堿?；D移酶和不依賴輔酶A的轉?；傅幕钚?。

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Alteration of membrane phospholipid fatty acid compositions has been shown to be important for leukocyte inflammatory responses. Such modification of the molecular species of these lipid classes requires deacylation and reacylation reactions and for phosphatidylcholines, lysophosphatidylcholine acyltransferase (LPCAT) and a coenzyme A-independent transacylase (CoAIT) can each be involved. Since previous studies have shown a significant IFNγ- and TNFα-induced modification of phosphatidylcholine species, we have examined whether these inflammatory cytokines alter the activity of reacylation enzymes in the human monocyte cell line MonoMac 6 (MM6). IFN-γ caused a significant increase in the activity of the LPCAT and CoAIT enzymes in the microsomal fraction at concentrations and over a time-course consistent with an important role for these enzymes in the sensitization (priming) of monocytes. In contrast, TNFα was found to significantly increase the activity of the CoAIT by 50% over controls in MM6 cells after 30 min incubation with the cytokine, but decreased LPCAT activity by 65% after 24 h incubation. Such data imply that CoAIT is important for the remodelling of phospholipid composition, which is seen during the acute response of cells to TNFα. The results provide further information to emphasise the role of acyltransferases as part of the molecular mechanism underlying inflammation. © 2005 Elsevier B.V. All rights reserved.
機譯：膜磷脂脂肪酸組成的改變已顯示對于白細胞炎癥反應很重要。這些脂質種類的分子種類的這種修飾需要脫?；驮脔；磻?，并且對于磷脂酰膽堿，可以分別涉及溶血磷脂酰膽堿?；D移酶（LPCAT）和不依賴輔酶A的轉?；福–oAIT）。由于先前的研究顯示了IFNγ和TNFα誘導的磷脂酰膽堿物種的顯著修飾，因此我們已經檢查了這些炎性細胞因子是否會改變人單核細胞系MonoMac 6（MM6）中再?；傅幕钚?。 IFN-γ導致微粒體級分中的LPCAT和CoAIT酶的活性在一定濃度和時間范圍內顯著增加，這與這些酶在單核細胞致敏（引發）中的重要作用一致。相反，在與細胞因子孵育30分鐘后，發現TNFα比MM6細胞中的對照顯著提高CoAIT活性50％，但在24小時孵育后使LPCAT活性降低65％。這樣的數據表明，CoAIT對于磷脂組成的重塑很重要，這在細胞對TNFα的急性反應中可見。結果提供了進一步的信息，以強調?；D移酶作為炎癥分子機制的一部分的作用。 ＆復制; 2005 Elsevier B.V.保留所有權利。

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