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Diversity of SREBP-1 gene expression in rat adipose tissue depots in response to refeeding after food restriction

機譯:食物限制后補充食物對大鼠脂肪組織貯庫中SREBP-1基因表達的多樣性

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The SREBP-1c mRNA level and precursor (microsomal) form of SREBP-1 abundance were significantly higher in epididymal and perirenal than in subcutaneous white adipose tissue of control rats. Moreover, the SREBP-1c mRNA level and an amount of precursor form of SREBP-1 were significantly higher in the epididymal and perirenal white adipose tissue of rats rnaintained on restricted diet and refed ad libitum for 48 h as compared to the control animals. No significant effects of food restriction/refeeding on SREBP-1c mRNA level and an amount of precursor form of SREBP-1 were found in subcutaneous white adipose tissue. The mature (nuclear) form of SREBP-1 was significantly increased in the epididymal, perirenal and subcutaneous white adipose tissue of the food restricted/refed animals. The activity, protein level and the mRNA abundance of malic enzyme (one of the target genes for SREBP-1) increased significantly in the epididymal, perirenal and subcutaneous white adipose tissue of the food restricted/refed rats as compared to the control animals, however the increase in perirenal and epididymal was higher than in the subcutaneous white adipose tissue.The results presented suggest that SREBP-1c is differently expressed in various rat white adipose tissue depots both under basal (control) and dieting conditions. © 2004 Elsevier B.V. All rights reserved.
機譯:附睪和腎周的SREBP-1c mRNA水平和SREBP-1豐度的前體(微粒體)形式明顯高于對照組大鼠的皮下白色脂肪組織。此外,與限制動物相比,在限制飲食和隨意進食48 h的大鼠的附睪和腎周白色脂肪組織中,SREBP-1c mRNA的水平和SREBP-1的前體形式的量顯著更高。在皮下白色脂肪組織中,未發現限制食物/補充食物對SREBP-1c mRNA水平和SREBP-1前體形式的影響。 SREBP-1的成熟(核)形式在禁食/禁食動物的附睪,腎周和皮下白色脂肪組織中顯著增加。與對照動物相比,食物受限/再攝取大鼠的附睪,腎周和皮下白色脂肪組織中蘋果酶(SREBP-1的靶基因之一)的活性,蛋白質水平和mRNA豐度顯著增加,但是腎周圍和附睪的增加高于皮下白色脂肪組織。提出的結果表明,在基礎(對照)和飲食條件下,SREBP-1c在各種大鼠白色脂肪組織貯庫中均有不同的表達。 &復制; 2004 Elsevier B.V.保留所有權利。

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